327216_2009_06_f[1] (2) - UNIVERSITY OF THE WESTERN CAPE...

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Unformatted text preview: UNIVERSITY OF THE WESTERN CAPE FINAL EXAMINATION MAY / JUNE 2009 MODULE NAME : BIOTECHNOLOGY 216 (BIOMOLECULES) MODULE CODE : 327216 DURATION : 3hrs MARKS : 150 STUDENTS ARE REMINDED TO CONSULT THE DEPARTMENTAL NOTICE BOARDS TO ASCERTAIN WHETHER THEY QUALIFY FOR A RE- EVALUATION OR SPECIAL EXAMINATION IN A PARTICULAR MODULE. Read the instructions carefully There are THREE sections Answer all questions in Section A using the form provided. Answer all questions in Section Band C using the exam books provided. SECTION A: MULTIPLE CHOICE QUESTIONS (No negative marking applies): 1. Biological function is achieved through mechanisms based on: 1. structural identity. 2. rigid, static lattices. 3. weak chemical interactions. 4. structural complementarity. 4 only I and 2 only 1 and 3 oniy 3 and 4 only 9.0 9‘9: 9. a b c d Insoluble in water. Roughly spherical. Hydrophobic side chains are exposed to the water. All are true Membrane proteins differ from globular proteins in that: a b c d 10. 11. 12 14 15 membrane associated amino acids usually have poiar side chains. membrane proteins are much more soluble in detergents than water. membrane proteins usually have more hydrophobic amino acids. All are true. is specific in hydrolyzing only peptide bonds in which the carboxyl function is contributed by an arginine or a lysine residue. a. Chymotrypsin b. Carboxypeptidase c. Trypsin d. CNBr or—Keratin has all of the following characteristics EXCEPT: a. primary component in hair, claws, fingernails. and horns of animals. b. consists of four helical strands arranged as twisted. pairs of two— stranded coiled coils. c. has associated hydrophobic strips on the two coiled coils. d. principal constituent of connective tissues in animals. . Silk fibers consist of .. 9—.“ 9‘?” proteins consisting of alternating and or “ residues. fibroin; glycine; proline; leucine a—keratin; alanine; glycine; serine fibroin; glycine; alanine; threonine fibroin; glycine; alanine; serine . Carbohydrate characteristic chemical features include all EXCEPT: 9.0.5?” the existence of one or more asymmetric centers. the capacity to form polymeric structures. the ability to exist in either linear or ring structures. all are true. . Oligosaccharides include all EXCEPT: 9‘99“?” monosaccharides. disaccharides. trisaccharides. pentasaccharides. .Lipids are the biomolecules of choice for storage of metabolic energy because they : 22. uses seven of the same enzymes as glycolysis, but must replace three enzymes of glycoiysis because they are a b. c. d. e. Catabolism; to oxidative Gluconeogenesis; irreversible Oxidative phosphorylation; reductive Giuconeogenesis; anabolic Oxidative phosphorylation; irreversible 23. The function of NADPH is to: 9999‘?” provide reducing equivalents to synthesize glucose from C02. carry electrons from anabolic reactions. provide a source of hydrogens for oxidative biosynthesis. be reduced by hydride ions to form NAD"l". produce an oxidative product. 24. For phosphofructokinase- 1: new» 6. Low ATP stimulates the enzyme, but fructose-2,6~bisphosphate inhibits. High ATP stimulates the enzyme, and fructose—2:6~bisphosphate activates. High ATP stimulates the enzyme, but fructose—2,6—bisphosphate inhibits. The enzyme is more active at low ATP than at high, and fructose—2.6— bisphosphate activates the enzyme. ATP and fructose—2.6—bisphosphate both inhibit the enzyme. 25. In the TCA cycle, carbon enters the cycle as and exits as 51°53‘ng 26. The sir-299‘s __m with metabolic energy captured as , and malonate; water; NADH; ATP; NADPH acetyi—COA; C02; NADH; ATP; NADPH succinyi»CoA; C02; ATP; NADH; NADPH acetyl~CoA; (30;; ATP; NADH; {FADHZ} malonyl—COA; water; NADH; [FADHZE ATP of pymvate to acetyl—COA is catalyzed by dehydration; pyruvate dehydration complex oxidative decarboxylations; pyruvate dehydrogenase complex decarboxylations; pyruvate decarboxylase transacylation; pyruvate transacylase none of the above. 27. Which enzymes of the TCA cycle cataiyze oxidative decarboxylation reactions? was???» malate dehydrogenase and citrate synthase fumarase and succinate dehydrogenase a-ketoglutarate dehydrogenase and succinate dehydrogenase isocitrate dehydrogenase and OL-ketoglutarate dehydrogenase aconitase and succiuate dehydrogenase SECTION B: Answer all questions in the exam hook provided [45 marks} l. The pH of a 0.02 M solution of an acid was measured at 4.6. Calculate the acid dissociation constant Ka. (6) 2. Calculate the pH at which the ot-arnino group of Eysine (pKamlOS) is 20% dissociated. (8) 3. Write the equilibrium constant, Keg, for the hydrolysis of creatine phosphate and calculate a value for Kt;q at 25 C’( AGE” = 43.3 lei/incl) (6) 4. List the weak interactions responsible for maintaining protein conformation. (4) 5. Proteins composed predominantly of b-pleated sheets may be expected to form structures that are flexible yet inextensible. Please explain. (3) 6. Amylose and cellulose are both plant polysaccharides composed of glucose in 1W4 linkage. However, amylose is a storage polysaccharide that is readily digested by animals whereas celluiose is a structural polysaccharide that is not digested by animals. What difference in the two polymers accounts for this? (5) 7. What group of amino acids do Histidine and Aspartic acid. belong to? Draw the structure of their R-groups (side chains). (8) 8. What are the properties of a ocuhelix? How would you describe a helix (what formula will you use)? (5) ...
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