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Unformatted text preview: 2009 Poultry Science 88 :1216-1226 doi: 10.3382/ps.2008-00452 Key words: broiler , amino acid , glycine , low crude protein ABSTRACT Research was conducted to determine the level of l-Lys that can be included in corn-soybean meal (C-SBM) diets for broilers before an amino acid (AA) beyond Met, Lys, Thr, or Gly becoming limit- ing and to determine the order of limiting AA in low CP C-SBM diets. All experiments were conducted with Ross 708 broilers (0 to 18 d of age) in brooder batter- ies. Treatments contained 7 or 8 replicates with 6 birds per replicate. In all experiments, a control C-SBM diet containing no l-Lys·HCl and a similar diet [positive control (PC) + Gly] with supplemental Gly to provide 2.32% total dietary Gly + Ser were fed. All diets were formulated to contain 1.26% total Lys. All diets with added l-Lys·HCl contained supplemental Gly to pro- vide 2.32% total dietary Gly + Ser. In experiment 1, l-Lys·HCl was added to the diets at 0.02% increments from 0.15 to 0.27%. Compared with the PC + Gly diet, there were no negative effects ( P &gt; 0.10) of supple- mental Lys on ADG, ADFI, or G:F. In experiment 2, l-Lys·HCl was added to the diets at 0.05% increments from 0.25 to 0.60%. Compared with the PC + Gly diet, ADG and G:F were decreased ( P &lt; 0.03) in broilers fed diets containing greater than 0.30% l-Lys·HCl but not ( P &gt; 0.10) in the 0.25% l-Lys·HCl diet. In experiment 3, l-Lys·HCl was added to the diets at 0.05% incre- ments from 0.20 to 0.30%. Daily gain was decreased ( P &lt; 0.03) in broilers fed 0.30% l-Lys·HCl but not in those fed 0.20 or 0.25% l-Lys·HCl. In experiment 4, the order of limiting AA was determined in a C-SBM diet containing 0.45% L-Lys·HCl. In addition to the PC and PC + Gly diets, diets consisted of a negative control (NC) diet with 0.45% l-Lys·HCl, NC + 0.247% Ile, NC + 0.484% l-Arg·HCl, NC + 0.249% Val, and all possible 2- and 3-way combinations of all 3 AA. Compared with the NC diet, addition of Arg and the combination of Arg and the other AA increased ADG and ADFI, indicating that Arg was the limiting AA in this diet. Experiment 5 was conducted in an identi- cal manner to experiment 4 except the diets with the added AA contained the same ratio of corn to soybean meal that is present in a diet with 0.25% l-Lys·HCl. The results of experiment 5 suggest that Arg and Val are equaling limiting in a diet with 0.25% l-Lys·HCl. In summary, 0.25% l-Lys·HCl can be added to C-SBM diets supplemented with Met, Thr, and Gly with no negative effects on growth performance, and Arg and Val are equaling limiting (after Met, Thr, Lys, and Gly) in diets containing 0.25% l-Lys·HCl. Effect of incremental levels of L-lysine and determination of the limiting amino acids in low crude protein corn-soybean meal diets for broilers 1 A. M. Waguespack ,* S. Powell ,* T. D. Bidner ,* R. L. Payne ,† and L. L. Southern * 2 * School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge 70803; and † Evonik-Degussa Corporation, Kennesaw, GA 30144...
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This note was uploaded on 07/28/2011 for the course VET 4335 taught by Professor Sakomura during the Spring '11 term at University of South Pacific.
- Spring '11