lec09.spectroscopy

lec09.spectroscopy - UV/VISIBLE SPECTROSCOPY UV/VIS...

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UV/VISIBLE SPECTROSCOPY
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UV/VIS Spectroscopy What does a UV/Vis Spectrometer Measure?
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Transmittance (T) is defined as the amount of light passing through the sample solution (P) divided by the amount of incident radiation (Po) T = P/P o In practice P o is estimated by a “blank” which accounts for reflection and scattering losses. UV/VIS Spectroscopy
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UV/VIS Spectroscopy As the radiation is absorbed in the sample, the total intensity of radiation is reduced as it travels through the sample. This results in a non-linear relationship between transmittance and concentration.
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Absorbance (A) is based on the amount of light absorbed by the solution and is defined as the log of the inverse of the transmittance. A = log 10 (1/T) UV/VIS Spectroscopy
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UV/VIS Spectroscopy Within limits, the relationship between absorbance and concentration is linear.
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A = ε bc ε is the molar absorptivity with units of (L/mol-cm) b is the path length of the sample ie. the inside
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lec09.spectroscopy - UV/VISIBLE SPECTROSCOPY UV/VIS...

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