lec10.intro_chrom - INTRODUCTION TO CHROMATOGRAPY HISTORY...

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INTRODUCTION TO CHROMATOGRAPY
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HISTORY The Russian botanist Mikhail Tswett coined the term chromatography in 1906 to describe his experiments in separating different colored constituents of leaves by passing an extract of the leaves through a column
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Analytic technique to discover chemical components : a method of finding out which components a gaseous or liquid mixture contains that involves passing it through or over something that absorbs the different components at different rates Chromatography Web Dictionary:
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Chromatography Carrier Gas Flow Control Injector Port Column Column Oven Detector Recorder
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CHROMATOGRAPHY COLUMNS Packed Column: Typical HPLC columns but some gas chromatography columns also (especially older columns). The columns are packed with tiny particles. Capillary Column: Typical gas chromatography column which consists of a small diameter tube coated on the inside with stationary phase.
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CHROMATOGRAPHY THEORY
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PARTITION COEFFICIENT K = Co/Cw Co is concentration in the organic phase (solvent) Cw is the concentration in the aqueous phase (water) Remember from the solvent lecture……….
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K = Co/Cw Co is concentration in the organic phase (solvent) Cw is the concentration in the aqueous phase (water) PARTITION COEFFICIENT molar concentration in stationary phase molar concentration in mobile phase K =
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PARTITION COEFFICIENT ETC. mass in the stationary phase mass in the mobile phase volume of mobile phase volume of stationary phase concentration in stationary phase concentration in mobile phase K = k = b =
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PARTITION COEFFICIENT ETC. k = K/b If mass = volume x concentration then:
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EXAMPLE: K = 4 b = k = grams in mobile phase = Compound A: mass = 1 mg Vol. Mobile Phase: 1 mL Vol. Stationary Phase: 1 mL Compound A: mass = 1 mg Vol. Mobile Phase: 1 mL Vol. Stationary Phase: 2 mL
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lec10.intro_chrom - INTRODUCTION TO CHROMATOGRAPY HISTORY...

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