Mod2Lab.xlsx - [S Rate 1[S 10 15 20 25 30 45 60 0.6 0.75...

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[S] Rate 1/[S] 1/V [S] 10 0.6 0.1 1.666667 5 15 0.75 0.066667 1.333333 10 20 0.9 0.05 1.111111 15 25 1 0.04 1 20 30 1.1 0.033333 0.909091 25 45 1.2 0.022222 0.833333 30 60 1.25 0.016667 0.8 35 Enzyme: 1/Vmax= 0.586 Vmax= 1.706 Km=18.411 Km/Vmax=10.789 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08 0.09 0.1 0.11 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 f(x) = 10.7890648669206 x + 0.586447399614811 R² = 0.99337024851865 Question 7: Lineweaver Burk Plot for Enzyme 1/[S] 1/V 0. 0 2 4 6 8 10 12 1/V 0 10 20 30 40 50 60 70 0 0.2 0.4 0.6 0.8 1 1.2 1.4 Question 7: Primary Plot for Enzyme Rate vs. [S] [S] Rate
Rate Enzyme 1/[S] Enzyme 1/V (Enzyme) Rate for Enzyme Inhibitor 1 0.2 0.2 5 0.1 0.35 0.1 2.85714285714286 0.17 0.5 0.06666666667 2 0.22 0.55 0.05 1.81818181818182 0.27 0.59 0.04 1.69491525423729 0.3 0.63 0.03333333333 1.58730158730159 0.35 0.64 0.02857142857 1.5625 0.4 Enzyme: Inhibitor 1 1/Vmax= 0.8435 1/Vmax=1.514 Vmax=1.186 Vmax=0.660 Km=24.268 Km=28.282 Km/Vmax= 20.471 Km/Vmax= 42.844 .02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 0.22 f(x) = 42.8443916971641 x + 1.51487285316266 R² = 0.996997765996683 f(x) = 20.4701509309211 x + 0.84354372936329 R² = 0.992102056303452 Question 8: Lineweaver Burk Plot Enzyme Inhibitor 1 is Noncompetitiv 1/[S] 0 5 10

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