Lab 1 Outline - Melissa Murano 1/29/08 Section R3 Lab 1...

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Melissa Murano 1/29/08 Section R3 Lab 1 Outline: Microscopy I. Review of Microscopy Best Practices a. Handle microscope with care, return to scanning lens and light intensity to lowest setting II. Parts of the Compound Light Microscop a. Four objective lenses: scanning (4x), lower-power (10x), high-power (40x), and oil immersion (100x). (shortest is the lowest magnification) III. Adjusting the Ocular lenses for your eyes a. Need a reptile blood slide, use scanning lens first IV. Calculating Total Magnification a. Determine total magnification by multiplying the magnification of the ocular lens (10x) by the magnification of the objective lens (4x, 10x, 40x, 100x) b. Always label drawings with the magnification V. Determining the diameter of the visual field a. Use grid on page 6 and circumscribe quarter then estimate diameter b. Take dime place in middle of drawn quarter and estimate diameter related to quarter then check accuracy by measuring the grid c. Obtain a grid slide and use scanning lens to bring into focus, use grid to estimate
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This note was uploaded on 04/05/2008 for the course CHEM 161, 101 taught by Professor Hansell during the Spring '08 term at Rutgers.

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Lab 1 Outline - Melissa Murano 1/29/08 Section R3 Lab 1...

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