Lab 11 GramNegative - E. aerogenosa was able to metabolize...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
BIO 233: RT Lab Report 11 : Gram Negative Biochemical Tests Objective : To have a full understanding of metabolism, growth and defenses of microorganisms. Results Table ANTIMICROBIAL E. aerogenosa E. coli P. mirabilis Sucrose A/G -/- K Glucose A/G A/G A/- Lactose A/- A/G K MR/VP - + + - + - Citrate + - + TSI A/A A/A/g+/H 2 S- K/K [sulfur (H 2 S) no fermentation] Nitrate - - + Urea - - + SIM Sulfur reduction + - + Indole - + + Motility + - + Phenyl alanine - - + MR/VP = methyl red/Voge’s Prauskauer SIM = sulfur indole motility TSI = triple sugar iron (aka Kligles Iron Agar) Conclusion:  E. aerogenosa was able to metabolize and create fermentation with both sucrose and glucose, while E. coli was able to metabolize and create fermentation with both glucose and lactose.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: E. aerogenosa was able to metabolize lactose but no fermentation was evident. P. mirabilis was able to metabolize glucose but no fermentation was evident. E. coli was positive for methyl red, TSI, and indole. E. aerogenosa was positive for Voge’s Prauskauer, citrate, and motility. P. mirabilis was positive for methyl red, citrate, nitrate, urease, sulfur reduction, indole, motility and phenyl alanine. Discussion: We had no problems with this lab, and all the tests came out as we expected. Furthermore, this lab enabled us to observe and better understand metabolic activity, growth and defenses of different microorgansims....
View Full Document

This note was uploaded on 08/02/2011 for the course MICRO 233 taught by Professor Diaz during the Fall '10 term at City Colleges of Chicago.

Page1 / 2

Lab 11 GramNegative - E. aerogenosa was able to metabolize...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online