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Unformatted text preview: 11-8-10
Lab: LR8 Standard Plate Count
Phase Dilution Factor Colonies—Sample 1 Colonies—Sample 2 Tube 2 10 -4 50 100 Tube 3 10 -5 TFTC TFTC Tube 4 10 -6 TFTC TFTC Tube 5 10 -7 TFTC TFTC Conclusion: The average number of colonies from Tube 2’s A1 and A2 plates was 75. The others were all
too few to count.
CSU= 75, FDF= 10 -4 ml
CSU= 75 x 10,000
The original density of E. coli in the broth was 750,000 CFU/ml
Discussion: The serial dilution only produced only one countable plate of colonies, in the first dilution plate.
Using parallel plates insured accuracy, and the same results were achieved on each set of plates. ...
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This note was uploaded on 08/02/2011 for the course MICRO 233 taught by Professor Diaz during the Fall '10 term at City Colleges of Chicago.
- Fall '10