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Unformatted text preview: BMS 500 MOLECULAR AND CELL BIOLOGY FALL 2010 Meets 9:00-10:50 AM Monday and Thursday Room 1041, David Axelrod Institute Course Description This course will provide in depth detail of essential cellular processes at the molecular level by using examples from the current literature. Topics to be covered trace the flow of information at the molecular level in cells. These include DNA structure and function, replication, DNA damage repair, transcription, posttranscriptional regulation and RNA interference, translation and relevant discussions of cell biology. An outline is presented below. Objectives Students will attain familiarity with basic concepts and some advanced material relevant to the topics to be covered (see Course Description). At the conclusion of the course, students will be able to understand and critique data from experiments in the relevant areas, and will be graded using exams that test their retention of basic material and their ability to apply it to real or simulated experimental data. Course Web Site Lecture and reading material will be made available on the course web site, which is on SUNY Albany’s electronic reserves Access requires a current UAlbany netID. The course is listed as BMS500. The password is: mcb10 Office hours M-F, 9-5; meetings should be arranged with individual instructors, as this is a team-taught course. Grading scheme A-E; three non-cumulative exams of equal weight. Course requirements Readings from the scientific literature will be assigned by individual instructors. Exams will be given on October 11th, November 11th, and Decmeber 16th. Exams are open book, but materials must be taken out of your bag or backpack before the beginning of the exam. Exam questions will primarily be short answers and short essays and will require problem solving. Course grades are determined based on exam performance or on homework assignments. Detailed syllabus: DNA structure: The content and complexity of the human genome will be examined. Genome size, unique and repetitive sequence content, and gene number and function will be discussed. Other topics include the increased genetic complexity provided by features of the proteome and the transciptome and human genetic variation and disease risk. Consequences of DNA mutation in human disease will be examined in discussion of transmission genetics and Mendelian inheritance and the analysis of human pedigrees. Nonmendelian and epigenetic inheritance will also be discussed. The structure and topology of DNA and the importance of chromatin structure is covered. DNA Replication. These lectures will cover the molecular mechanisms of DNA replication. Mechanisms of replication in bacteria and eukaryotes including detailed consideration of initiation and termination are covered. The replication of chromatin and the retention of epigenetic "marks" and developmental memory and telomere structure and replication are also discussed. DNA repair: The basic mechanisms of DNA repair in eukaryotes will be discussed. These will include how DNA damage occurs and how it is sensed. Mismatch repair, base excision repair, and UV excision repair mechanisms are detailed. Diseases associated with defects in DNA repair are discussed. The role of DNA recombination in repair is also covered. Topics include homologous recombination, non-homologous end joining (NHEJ), double strand break repair, biological and technological applications for recombination, model systems and recombination in health and diseases such as Nejmegen Brakeage Syndrome and Fanconi anemia. Transcriptional regulation: These lectures will begin with regulation of gene expression in phage lambda, which illustrates many basic principles. Eukaryotic transcription will be discussed, emphasizing transcription by polIII, mRNA transcription by RNA pol II, and current thoughts on how activators function. In addition, chromatin structure and function, topics of transcriptional repression, silencing, and regulated domains will be discussed, and an introduction will be given to chemical genetics. Post-transcriptional regulation: Topics covered include mRNA processing, nuclear export, mRNA stability and localization and translational regulation. Post-transcriptional mRNA editing will be offered. Emphasis will be placed on phenomenology and mechanism. Gene silencing: Topics covered will include: forms of gene silencing, the discovery of RNAi, RNAi mutants in C. elegans, RNAi biochemistry and the importance of microRNAs in development. Translation: The structure and function of the basic components that participate in protein synthesis will be introduced, including tRNA and ribosomes. The problems of tRNA identity and decoding the genetic code will be discussed. Impact of recent advancements in the structural studies of ribosome in understanding mRNA decoding on the small ribosomal subunit will be discussed. The four main steps in protein synthesis will be discussed. Molecular mimicry between tRNA and various protein factors will be outlined and discussed. The mechanism of action of several antibiotics in inhibiting the steps of translation will be discussed. Cell biology: A brief overview of cell structures with emphasis on the spatial and topological organization of the various membrane-bound intracellular compartments and the problems this creates for the movement of proteins between these compartments. Mechanisms for translocating proteins across intracellular membranes in trafficking proteins between intracellular compartments will also be discussed. Cell cycle: Topics covered will include: classical analysis of yeast cell cycle mutants, oocyte maturation, MPF, CSF, cyclins, and the convergence of these fields. The components of mammalian cell cycle machinery, common themes in cell cycle dysregulation in cancer, role of tumor suppressors in cell cycle regulation and checkpoints are also discussed. BMS 500 MOLECULAR AND CELL BIOLOGY FALL 2010 9:00-10:50 AM Room 1041, David Axelrod Institute Day Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Monday Thursday Date 30-Aug 2-Sep 6-Sep 9-Sep 13-Sep 16-Sep 20-Sep 23-Sep 27-Sep 30-Sep 4-Oct 7-Oct 11-Oct 14-Oct 18-Oct 21-Oct 25-Oct 28-Oct 1-Nov 4-Nov 8-Nov 11-Nov 15-Nov 18-Nov 22-Nov 25-Nov 29-Nov 2-Dec 6-Dec 9-Dec 13-Dec 16-Dec Lecture 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 finals Topic Course Introduction DNA structure & mutations No Class No Class DNA structure & mutations DNA structure & mutations DNA Replication DNA Replication DNA Replication DNA Repair DNA Repair Review EXAM 1 RNA transcription (Prokaryotic) RNA transcription (Prokaryotic) RNA transcription (Eukaryotic) RNA transcription (Eukaryotic) RNA transcription (Eukaryotic) RNA processing RNA processing Review EXAM 2 Protein translation Protein translation Gene silencing NoClass Cell Biology Cell Biology Cell Biology Cell Cycle Review EXAM 3 Instructor Conklin Glaser Glaser Glaser Wolfgang Wolfgang Wolfgang Begley Begley Lectures 1 - 9 Wade Wade Morse Morse Morse Madison-Antenucci Madison-Antenucci Lectures 10 - 18 Agrawal Agrawal Conklin Mazurkiewicz Mazurkiewicz Mazurkiewicz Conklin Lectures 21-27 Instructors: Doug Conklin Bill Wolfgang Bob Glaser Tom Begley Joe Wade Randy Morse Susan Madison-Antenucci Raj Agrawal Joe Mazurkiewicz CRC DAI CMS CRC CMS CMS DAI ESP AMC 591-7154 486-1156 473-4201 591-7154 474-5727 486-3116 408-2827 486-5797 262-5381 ...
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This note was uploaded on 08/03/2011 for the course SPH 592 taught by Professor Teamtaught during the Spring '11 term at SUNY Albany.

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