Lab 2 Enzyme Kinetics

Lab 2 Enzyme Kinetics - Lab #2 Enzyme Kinetics Where we are...

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1 Lab #2 Enzyme Kinetics Where we are going… ± Define… ± Enzyme ± Energy of activation ± Total energy change of a reaction ± Effect of temperature and pH ± Enzyme activity of LDH and its reaction Enzymes ± Biological catalyst 1. rate of reaction 2. does not change nature of reaction or the final product
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2 Energy of Activation… -Is the difference between E of transition state and E of R (or P) -Energy required for reaction to proceed Enzymes ± Biological catalyst 1. rate of reaction 2. does not change nature of reaction or the final product 3. not changed by the reaction • Substrate specific Lock and Key Model…. . The shape of the substrate “complements” the active site of the enzyme.
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3 Control of Enzyme Activity ± Temperature and pH ± Concentration of cofactors and coenzymes ± Concentration of enzyme and substrate ² Saturation ± Stimulatory and inhibitory effects of products ² Feedback Inhibition Effects of Temperature ± Temp = enzyme rate ² 0 ° to 40
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Lab 2 Enzyme Kinetics - Lab #2 Enzyme Kinetics Where we are...

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