Chem 30CL-Lecture 5a

Chem 30CL-Lecture 5a - Lecture 5a Click to edit Master...

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Click to edit Master subtitle style 8/6/11 Mass spectrometry Lecture 5a
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8/6/11 J. J. Thompson was able to separate two neon isotopes (Ne-20 and Ne-33) in 1913 (Noble Prize 1906, Physics, for the discovery of the electron) H. Dehmelt, W. Paul (Noble Prize 1989, Physics) built the first quadrupole mass spectrometer in 1953. K. Tanaka, J.B. Fenn (Noble Prize 2002, Chemistry) developed the electrospray and soft laser desorption method which are used for a lot of proteins History
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8/6/11 EI is hard ionization technique An ionizing beam of electrons generated in the ionization chamber causes the ionization and/or fragmentation of the molecule The higher the energy of the electrons is, the more fragmentation is observed up to the point where the molecular ion (M+) cannot be observed anymore Electron Impact Mass Spectrometry I Inlet vacuum Detector cathode anode 70 eV Magnetic Field (H) Sample Chamber Ionizing accelerator plates e - AB AB AB + AB + AB + AB + AB + AB + A + A + B + B + B +
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8/6/11
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Chem 30CL-Lecture 5a - Lecture 5a Click to edit Master...

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