RQ 1B - CHEN 4820 Reading Quiz RQ14 Lilie et al (protein...

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CHEN 4820 Reading Quiz RQ14 Lilie et al (protein refolding) Pledge: On my honor, I have neither given nor received aid on this quiz. Print name: _________________________________________ Signature: __________________________________________ Quiz: Q1. What kind of byproduct reaction is the primary cause of low product yield from a protein refold process? A Enzymatic clipping of product into fragments B Formation of product aggregates C Deamidation of the aspargine and glutamine residues of the product Ans = B During refolding, the partially folded product has exposed hydrophobic regions that can bind to hydrophobic regions of neighboring proteins, leading to aggregate formation. The aggregates never fully refold therefore represent lost yield (lost activity). The inclusion bodies are surprisingly pure, and enzymatic clipping is generally not a problem. Deamidation is a concern for the stability of protein formulations, but not at the cold temperatures typical of refolding. Q2.
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This note was uploaded on 08/10/2011 for the course CHEN 4820 at Colorado.

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RQ 1B - CHEN 4820 Reading Quiz RQ14 Lilie et al (protein...

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