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RQ9 - CHEN 4820 Reading Quiz RQ9(adsorption and...

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CHEN 4820 Reading Quiz RQ9 (adsorption and chromatography equipment and scaleup) Harrison sec 7.5 7.9 Pledge: On my honor, I have neither given nor received aid on this quiz. Print name: _________________________________________ Signature: __________________________________________ Quiz: Q1. Put “yes” next to the statements that give an example of a biospecific interaction between two molecules, something that could be the basis for designing an affinity chromatography resin. yes Enzyme and cofactor yes Receptor and agonist no Solvent and solute yes Antibody and antigen Q2. Put “yes” next to the statements that are true for size exclusion chromatography (SEC) yes SEC separates solutes on the basis of their size no In SEC, smaller molecules elute ahead of larger molecules yes SEC does not involve binding the solute to the resin Q3. In the context of chromatography, what is meant by non-specific binding? A Refers to the preparation of the resin whereby a ligand is bound to the resin support B Refers to the binding of solutes to the resin by a mechanism that was not intended C
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