RQ11 - C HEN 48 20 f all 2010 R eading Quiz R Q 1 1 S copes...

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CHEN 4820 fall 2010 Reading Quiz RQ11 Scopes sec 4.1-4.6 (precipitation) Pledge: On my honor, I have neither given nor received aid on this quiz. Print name: _________________________________________ Signature: __________________________________________ Quiz: Q1. Which technique is not generally used for precipitating a protein product because it carries too high a risk for denaturing the protein? A Isoelectric precipitation at low ionic strengths and room temperature B Precipitation by raising the solution temperature to >> 50 °C. C Precipitation with miscible organic solvents at COLD temperatures D Precipitation by the addition of lyotrophic salts at room temperature Ans = A or B. Organic solvents are a concern, but their denaturing tendencies can often be managed by operating at cold temperatures. A prominent example is the Cohn process for fractionating blood plasma proteins using ethanol at or near zero deg °C. (The ethanol nicely lowers the freezing point of the solution, allowing operation even below zero °C). Q2.
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RQ11 - C HEN 48 20 f all 2010 R eading Quiz R Q 1 1 S copes...

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