Harrison Chapter 8 Notes

Harrison Chapter 8 Notes - Chapter 8 Precipitation The...

Info icon This preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Chapter 8: Precipitation The process of coming out of solution as a solid Advantages: - Relatively inexpensive - Can be carried out with simple equipment - Can be done continuously - Leads to a form of the protein that is often stable in long-term storage 8.2 Protein Solubility Most important factors affecting the solubility of proteins: - Structure and size - Protein charge - Solvent Structure and Size - Forces acting on the protein lead to a minimization of the Gibbs free energy - Water solvation molecules form two distinct layers, known as hydration layers, around the protein molecule, the first totally oriented, the second mostly non-oriented - The size of the protein becomes important with respect to solubility when the protein is excluded from part of the solvent (i.e. when non-ionic polymers added to the solution result in steric exclusion of the protein molecules) Juckes model: where - S is the solubility, r s and r r the radius of the protein solute and polymer rod, Vbar partial specific volume, c p particle conc. and β’ is a constant
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern