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Unformatted text preview: Chapter 8: Precipitation The process of coming out of solution as a solid Advantages:- Relatively inexpensive- Can be carried out with simple equipment- Can be done continuously- Leads to a form of the protein that is often stable in long-term storage 8.2 Protein Solubility Most important factors affecting the solubility of proteins:- Structure and size- Protein charge- Solvent Structure and Size- Forces acting on the protein lead to a minimization of the Gibbs free energy- Water solvation molecules form two distinct layers, known as hydration layers, around the protein molecule, the first totally oriented, the second mostly non-oriented- The size of the protein becomes important with respect to solubility when the protein is excluded from part of the solvent (i.e. when non-ionic polymers added to the solution result in steric exclusion of the protein molecules) Juckes model: where - S is the solubility, r s and r r the radius of the protein solute and polymer rod, Vbar partial specific volume, c...
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This note was uploaded on 08/10/2011 for the course CHEN 4820 at Colorado.