Reading%20points%20for%20Harrison%20v%20100722

Reading%20points%20for%20Harrison%20v%20100722 - 02:00:43...

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02:00:43 CHEN 4820 - Reading points from Harrison textbook, Bioseparations Science and Engineering. Ch 1 – Intro to bioproducts and bioseparations Sec 1.0 Range of product volume and price in biotech. Dominant market sector. Sec 1.2 Range of bioproduct molecular weight. Huge variety of large molecules made from assembling a few different building blocks in different ways. Sec 1.3 Defn of primary metabolite, of secondary metabolite. Subclasses of primary metabolites. Subclasses of secondary metabolites. Sec 1.4 Commercial importance of macromolecules in biotech. Four levels of structure in protein molecule. Defn of primary structure, secondary structure, tertiary structure, quaternary structure. Defn of polypeptide. Denaturation and its causes. Defn of posttranslational modifications. Protein classes by type of prosthetic group. Basic structure of antibodies. Functional classes of proteins. Various ways proteins can degrade. Variables that bring about degradation.
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02:00:43 Idea of a fusion protein. Defn of inclusion body. Basic approach to solubilize and refold proteins at prodn scale. Defn of chaotrope. Defn of oxidation. Sec 1.5 Basic structure of a nucleotide. Basic structure of a nucleic acid. Relative stability of nucleic acids vs proteins. Emerging commercial uses of nucleic acids. Sec 1.6 Basic structure of a polysaccharide. Major uses of polysaccharides. Sec 1.7 Major classes of particulate products. Sec 1.8 Defn of genetic engineering. Bioprocess conditions to protect labile bioproducts Major stages of bioseparations, typical unit ops for each stage The Merck Index as a resource. Three major tools of engineering analysis for bioprocesses. Standard measures of product purity and activity. Standard defn of process yield. Sec 1.9
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02:00:43 The U.S. Pharmacopeia as a resource. The basic concepts of GLP and GMP. Defn of formulation Ch 2 - Analytical methods Sec 2.2 Defn of a specification for a product Major elements of a specification for a protein drug for a human. Ch 3 - Cell lysis and flocculation Intro Basic types of lysis methods Defn of flocculation Sec 3.2 Cell type vs lysis method used. Defn of prokaryotes. Basic structure of prokaryotes relevant to lysis. Basic differences between Gram negative vs Gram positive bacteria. Defn of eukaryotes, and basic structure relevant to lysis. Sec 3.3 Subclasses of mechanical and of chemical lysis methods. Principle of osmotic lysis. Formula for osmotic pressure. Types of chemical lysis methods. Types of mechanical lysis methods.
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02:00:43 Formula for degree of lysis using valve-type high pressure homogenizer. Principle of bead mill. Sec 3.4 Motivation for flocculation: increase particle size to allow filtration with less area or centrifugation with a smaller centrifuge to remove the particles. Principle of flocculation by manipulating the electrical charge of particles or the ionic strength of the
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Reading%20points%20for%20Harrison%20v%20100722 - 02:00:43...

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