MICR 21 Notes #2 - lab notes

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Lab 2b 1. Name and give the functions of the principal parts of your microscope. A: Oculars (eye piece 10x), head, coarse adjustment, fine adjustment, arm, nose piece, objectives, stage - holds slide, iris diaphragm lever -controls amount of light, condenser - focuses light, stage adjustment, light source/ Control light using: 1) condenser 2) iris diaphragm don’t use the rheostat 2. What is meant by the term parfocal? Resolution? Interpupillary distance? A: Parfocal – characteristic of objective lenses; focus on 10x using coarse and fine adjustments focuses when you move to 40x & 100x, you only need nminor adjustments to get it into focus Resolution - clarity to distinguish between two different points as separate points. Interpupillary distance - the distance between the oculars which must be set, depending on the distance between the pupils of the observer. 3. What is the purpose of immersion oil? A: Oil immersion is only used in the 100x or the objective with the greatest resolving power. Oil’s refractive index is the same as glass. It increases the numerical aperture value from that of air to oil; therefore, the resolution further increases. 4. What are the resolving power and the total magnification of each of the lenses of your microscope? A: 10x: rp- 0.2-2.3 micrometer mag- 100/ 40x: rp- … mag- 400/ 100x: rp- … mag- 1000 Lab 3 1. To which phyla do the organisms you observed today belong? Explain why you included them where you did? A: Paramecium bursaria belongs to phylum ciliaphora, Euglena gracilis belongs to the 2. What are pseudopodia? What functions do they perform? A: Pseudopodia literally translate to false feet. They are temporary projections of false feet which occur most commonly in Amoeba for motility. They extend and contract by the use of actin filaments. 3. Paramesium bursaria contains a unicellular green alga (Chorella paramecii) as a symbiont. Why do these organisms benefit from this arrangement? A: Lab 4
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1. Which of the organisms you observed today are eukaryotes? Which are prokaryotes? A: Hydrodicton sp. & Spirogyra sp. – eukaryote (green algae/ phylum: protists) Chlorella sp. – eukaryote (ss. plantae) Oscillatoria sp. – prokaryote (phylum: cyanobacteria) 2. What is the source of energy for these organisms? A: These organisms use light as their energy source. 3. Which of the organisms you observed today have a sexual cycle? Which have an asexual cycle? Both? 4. What mechanisms of motility are evident in the microbes you have observed so far? A: ciliaphora use cilia, Lab 5 1. Diagram and name the principal parts of the yeast cell. A: yeast contains the nucleus, outer cell wall made of chitin, some yeast also show signs of budding which is a form of asexual reproduction 2. Which of the yeasts we saw in lab today reproduce by budding? Which by binary fission?
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This note was uploaded on 08/10/2011 for the course MICR 21 taught by Professor Rech,sabine during the Summer '10 term at San Jose State University .

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MICR 21 Notes #2 - lab notes

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