BY 160 Respiration Formal Lab Report

BY 160 Respiration Formal Lab Report - BY 160 Respiration...

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BY 160 Respiration Formal Lab Report Introduction : The subject of the experiment was respiration. More specifically, the experiment was used to measure the relative production of carbon dioxide by aerobic organisms. Basically, cellular respiration converts energy obtained from organic molecules into energy that is usable by an organism, usually to be stored in the chemical bonds of ATP (Freeman 178). Through a series of reactions, respiration in aerobic organisms involves oxygen gas and organic carbon molecules being taken in and water and carbon dioxide being released as waste. In water, the carbon dioxide released can react to form carbonic acid which lowers the pH of the solution. In the experiment, the formation of carbonic acid from carbon dioxide and water is used to measure the production of carbon dioxide by a respiring organism. Through the use of the pH indicator, phenolphthalein, the relative respiration rate of a respiring organism could be calculated by measuring the volume of NaOH required to neutralize the carbonic acid produced (Vodopich &Moore 118). To further focus on just the production of carbon dioxide, the solution containing elodea was covered by a coffee can to prevent it from undergoing photosynthesis and skewing our results. The hypothesis tested for the experiment was that an animal, in this case a fish, respires more than a plant, elodea. The null hypothesis was that an animal does not respire more than a plant. Materials and Methods :
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The materials required for the experiment included: 225 mL of culture solution (dechlorinated water) 3 250-mL beakers 1 small fish 1 small elodea 1 50-mL graduated cylinder 3 pieces of plastic film (parafilm)
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This note was uploaded on 04/05/2008 for the course BY 160 taught by Professor Mckelvey during the Fall '07 term at Aquinas.

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BY 160 Respiration Formal Lab Report - BY 160 Respiration...

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