Lecture-17-10-21-10_44462

Lecture-17-10-21-10_44462 - Genomes and Genomics Human...

Info iconThis preview shows pages 1–24. Sign up to view the full content.

View Full Document Right Arrow Icon
Genomes and Genomics
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Human chromosomes range in length from 51 million to 245 million base pairs. If all the bases in the human genome were spread out 1 millimeter apart, they would extend from Memphis to Los Angeles.
Background image of page 2
The human nuclear genome viewed as a set of labeled DNA Chapter 13 Opener Each human cell has two sets of 22 autosomes and a set of sex chromosomes. A normal female will have a pair of X chromosomes; a male will have an X and Y pair.
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Chih-Lin Hsieh, Molecular & Clinical Cytogenetics Laboratory, Stanford University Medical Center
Background image of page 4
Human Genome: Important Numbers 1. 23 chromosomes in haploid genome. 1. ~3 billion (3 x 10 ) base pairs of DNA in haploid
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Neanderthal bone sample for DNA sequencing Figure 13-1
Background image of page 6
Genome sequencing is now automated Figure 13-3
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Two possible approaches to sequence a genome: Whole-genome shotgun approach works best for simpler genomes sequence first, then bring sequences into order Order clones first: Ordered clone approach
Background image of page 8
Figure 13-2 step 1 The logic of creating a shotgun sequence map of the genome
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 13-2 step 2 The logic of creating a shotgun sequence map of the genome
Background image of page 10
and other enzymes
Background image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 13-2 step 3 The logic of creating a shotgun sequence map of the genome
Background image of page 12
Figure 13-2 step 4 The logic of creating a shotgun sequence map of the genome
Background image of page 13

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 13-2 step 5 The logic of creating a shotgun sequence map of the genome
Background image of page 14
Complications for either method, but especially for the shotgun method are repetitive sequences that are present in the genome in high numbers with very similar sequences
Background image of page 15

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
About 50% of the Human Genome is  Comprised of Transposable Elements LINEs : L ong in terspersed e lements SINEs : S hort in terspersed e lements
Background image of page 16
Producing overlapping clones for sequencing http://genomics.energy.gov Plasmids that carry the inserts are called vectors
Background image of page 17

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 13-8 step 1 Strategy for ordered-clone sequencing
Background image of page 18
Figure 13-8 step 2 Strategy for ordered-clone sequencing
Background image of page 19

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
A physical map puts clones in order Figure 13-7b
Background image of page 20
Figure 13-8 step 3 Strategy for ordered-clone sequencing
Background image of page 21

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 13-8 step 4 Strategy for ordered-clone sequencing
Background image of page 22
Figure 13-8 step 5 Strategy for ordered-clone sequencing
Background image of page 23

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 24
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 08/16/2011 for the course BIOL 1361-1362 taught by Professor Any during the Spring '08 term at University of Houston.

Page1 / 54

Lecture-17-10-21-10_44462 - Genomes and Genomics Human...

This preview shows document pages 1 - 24. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online