Unformatted text preview: 2. Apply fluorescently labeled antibodies to skin specimens to detect presence and pattern of cell-specific markers. (2H3 for nerves = green; PECAM-1 for undifferentiated endothelial cells = blue). 3. Wash away any unbound antibody. 4. Examine labeled skin whole mount samples by confocal fluorescence microscopy. 5. Compare staining patterns of nerves and blood vessels . b. Describe the key RESULTS of this experiment. The pattern of arterial cell staining mimics that of peripheral nerves (Fig. 1abc). The pattern of venous cell staining does not mimic peripheral nerve pattern (Fig. 1ghi) c. Do these results establish plausibility, necessity, or sufficiency? Explain the basis for your choice. It is plausible that arteries and neurons influence each other. A correlation in space has been established, but no proof of cause and effect....
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- Spring '08
- Blood vessel, mouse embryo limb, embryo limb skin