bis_104_pq_5__ans_spring_08 - stain for peripheral...

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BIS 104 PQ 5 ans spring 08 5. To further investigate the possible role of peripheral nerves in mouse limb skin arteriogenesis, Mukouyama made use of the Neurogenin 1 / Neurogenin 2 (Ngn1 / Ngn2) double homozygous mutant strain of mice. It had been established previously that these mice lack peripheral sensory nerves. Describe the experiments that Mukouyama did with these mice. Embryonic limb skin samples from both normal (wild type) and Ngn mutant mice were
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Unformatted text preview: stain for peripheral nerves (2H3 = green fluor); undifferentiated ECs (PECAM-1 = blue fluor.), and arteries (ephrinB2 7 CX40 = red fluor.). Both tissues were examined by scanning confocal fluor. microscopy. The staining patterns were compared (normal vs. mutant) What conclusion did he arrive at based on the results of these experiments? Peripheral sensory nerves are necessary (required) for arterial differentiation ....
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