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Unformatted text preview: the other cell type. Both populations were shown to express mRNA for VEGF and for HGPRT as expected. b) The PCR –notherern blot results were confirmed by examining the two populations purified by FACS by immunocytochemistry. Fluoresently labeled antibodies for both cell types were applied to both populations. Examination by fluorescence microscopy showed that the populations were > 95% “pure”, that is, little or no cross-contamination of cell types. So, yes, the FACS technique successfully separated neurons from Schwann cells in this experiment....
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This note was uploaded on 08/18/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08