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Unformatted text preview: Cells from normal individuals show positive plasma membrane labeling, whereas the FH-fibroblast lack labeling. (alternative experiment: Isolate plasma membranes from both cell types by differential centrifugation; verify purity by TEM of samples. Separate PM proteins by SDS-PAGE; transblot (Western) separated proteins and probe both samples with a labeled antibody specific for LDL receptor protein. Results as above: PM extracts from normal cells are positive; FH- cells are negative...
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- Spring '08