Walters-BSC2010-Chapter16-10

Walters-BSC2010-Chapter16-10 - Dr. Linda Walters Room:...

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Dr. Linda Walters Room: Biology 401C Phone: 407-823-2148 email: ljwalter@mail.ucf.edu Office hours: Tuesday 9:00 AM – 11:00 AM Wednesday: 2:30 – 4:00 PM Friday: 2:30 – 4:00 PM or by appointment
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Course Information Available now on Webcourses: 1) All powerpoint slides by chapter. 2) Practice test questions available ~ 1 week before tests. You are responsible for all materials covered in class (includes materials NOT in text book). Tests: focus on book content that compliments class materials (You will NOT be tested on text material not covered in class).
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BSC 2010 Faculty Professor Thomas Tall Not so loud Deal with all concerns through Exam 3 and SARC 5 bonus points in lecture Dr. Walters Less tall Can be very loud Deal with all concerns from November 3 on (except SARC) 5 bonus points in lecture, everything else the same
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Dr. Linda Walters Human impacts on the marine environment Coral reefs Seagrasses Mangroves Invasive species Oyster reefs
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Chapter 16: The Molecular Basis of Inheritance Need to know: Scientists’ biographies, their experiments in detail, DNA replication
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DNA Substance of inheritance Located on chromosomes Polymer composed of monomers called nucleotides Each nucleotide contains: phosphate group pentose sugar deoxyribose nitrogenous bases
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Fig. 16.5
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~60 years ago, none of this known. Huge challenge to: 1) determine substance of inheritance & 2) it’s 3-D molecular structure.
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Know in ~1950: Hereditary material on chromosomes Chromosomes composed of protein and DNA Most thought proteins contained the genetic material (more diverse molecules)
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1952: Best evidence to date that DNA was genetic material Studied bacteriophage T2 bacteriophage = virus that infects bacteria (also called phage) virus = DNA surrounded by protein coat Chase Hershey
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Bacteriophage T2 T2 infects bacteria E. coli T2 quickly reprograms E. coli to produce T2 phages, released when cell lyses
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HERSHEY AND CHASE Question : Which viral component was responsible for reprogramming the host bacterial cell? The protein or the DNA? Answered question by devising experiment that showed that only one component actually enters E. coli during infection
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Hershey and Chase Experiments: Step 1: In separate dishes, label viral protein and viral DNA tagged with different radioactive isotopes proteins tagged with radioactive sulfur DNA tagged with radioactive phosphorous
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Fig. 16.4
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Hershey and Chase Experiment: Step 2a: Allow protein labeled phages to infect E. coli Step 2b: Allow DNA labeled phages to infect E. coli Step 3: Agitate cultures (blender), this shakes lose phage components outside the bacterial cells
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Fig. 16.4
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Walters-BSC2010-Chapter16-10 - Dr. Linda Walters Room:...

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