8 enzyme kinetics

8 enzyme kinetics - Chemical kinetics A B v = d[A dt = k[A...

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Chemical kinetics A B v = d[A] / dt = k[A] first order A + B C v =d[A][B] / dt = k[A][B] second order K in terms of sec -1
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Reaction rate increases As [enzyme] increases
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During the course of an enzyme catalyzed reaction, the [substrate] decreases with time, and the [product] increases with time
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E + S Substrate E S E + S* E + P Products Active site An enzyme catalyzed reaction
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When [S] >> [E], then ES is formed rapidly and stays relatively constant throughout the course of the reaction. This is referred to as steady state conditions.
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The equation for an enzyme catalyzed reaction can be written k 1 k 2 E + S ES E + P k -1 The formation of product can be expressed as the rate equation v o = k 2 [ES] If all of the enzyme were complexed with substrate, then maximal velocity is defined as V max V max = k 2 [E T ] Where E T is total amount of enzyme, and E T = E + ES Or ES = E T -E
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If we know [ES], we could figure stuff out. Let’s go back to the course of an enzyme catalyzed reaction and look at
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8 enzyme kinetics - Chemical kinetics A B v = d[A dt = k[A...

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