Cellular Respiration Lab Report

Cellular Respiration Lab Report - Josh Laster Biology 111...

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Josh Laster Biology 111 – Section: 523 10 – 06 – 10 The Aerobic Respiration Experiment Introduction: All Eukaryotic cells require oxygen for aerobic respiration. Oxygen is mainly used near the end of cellular respiration, where most of ATP is produced (oxidative phosphorylation). Glycolysis (occurs in the cytosol) starts off cellular respiration by oxidizing a glucose molecule in a series of ten enzyme facilitated steps into two pyruvate, a net of two ATP, two NADH plus two hydrogen ions, and two water molecules. The pyruvate then enters the mitochondria and a carboxyl group is removed creating carbon dioxide as a waste product. Acetate is then formed by the remaining two-carbon group and NAD is reduced to NADH. Acetyl CoA is formed when coenzyme A (an organic cofactor) bonds to acetate. Acetyl CoA enters the Krebs cycle (known as citric acid cycle) and forms citrate when added to oxaloacetate. FADH2 and NADH (both electron carriers) facilitate the reaction cycling back to oxaloacetate. The citric acid cycle has an output of four carbon dioxide, two FADH2, two ATP, six NADH plus six hydrogen ions, and two coenzyme A. All the FADH2 and NADH from the Krebs cycle and glycolysis transport elections to the electron transport chain. While electrons go towards oxygen (at the end of the chain), Hydrogen ions get pumped into the intermembrane space. These hydrogen ions are then
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