Finalexam153CF02 - 1 FROM CH C.F CLARKE 2A Dept of Chemistry and Biochemistry University of California Los Angeles 607 Charles E Young Drive East

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1 FROM: C.F. CLARKE CH- 2A Dept. of Chemistry and Biochemistry University of California, Los Angeles 607 Charles E. Young Drive East Los Angeles, CA 90095-1569 CH-2A TO:
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Chemistry & Biochemistry 153C Fall 2002 Name____________________________________ Student I.D. #____________________________ FINAL EXAMINATION 10 December 2002 Total Score and Grade on Final _____________ (150 points, 7 pages including cover page, 180 minutes) Total Score in Class _____________ Grade in Class _____________ PLEASE READ EACH QUESTION VERY CAREFULLY! POTENTIALLY USEFUL CONSTANTS: R = 0.0083 kJ mol -1 ˚K -1 ; Ambient temp =298˚K; F = 96.5 kJ mol -1 volt -1 ; 1 kcal = 4.2 kJ 1. (20 points total) (part a) (5 points) In this class, it has been stated that the NAD + /NADH ratio inside cells is maintained at 100:1. For mammalian cells, local [NAD + ]/[NADH] ratios have been estimated indirectly by measuring the product-substrate ratios of NAD + -dependent dehydrogenases confined to specific compartments. Lactate dehydrogenase, is a cytoplasmic enzyme that has been used for this type of analysis. Write out the reaction catalyzed by lactate dehydrogenase. (Either names or structures may be used). (part b) (10 points) The Δ G ° for formation of lactate in the reaction catalyzed by lactate dehydrogenase is -25.1 kJ mol -1 . Describe how the ratio of NAD + /NADH could be determined. Be sure to state any assumptions that need to be made.
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This note was uploaded on 08/26/2011 for the course CHEMISTRY Chem 153C taught by Professor Cathyclarke during the Spring '11 term at UCLA.

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Finalexam153CF02 - 1 FROM CH C.F CLARKE 2A Dept of Chemistry and Biochemistry University of California Los Angeles 607 Charles E Young Drive East

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