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Unformatted text preview: Name_A_rlé_viQV___lé_’Li_________ 1 Chemistry & Biochemistry 153C 2nd MIDTERM EXAMINATION 15 May 2009 PDT: (90 points, 3 pages, 50 minutes) PLEASE READ EACH QUESTION VERY CAREFULLY! POTENTIALLY USEFUL CONSTANTS: R = 0.0083 kJ ' mol‘1 - °K'1; Pho‘m’uml Ambient temp = 298°K; F = 96.5 kJ ° mol‘1 ° volt'l; 1 kcal = 4.2 kJ; h = 6.6x10'3‘J - s; = 3x108 m ' s'1 E = LC. A REDUCTION POTENTIALS: E°' (Volts) 02 + 4e' + 451* 9 2 H20 0.815 PQ + e‘ 9 PQ" —o.o36 PQ" + e‘ + 2H+ 9 PQHZ 0.200 Plastocyanin(Cu2+)+ e' 9P1astocyanin(Cu1+) 0 .340 Cyt f(Fe3+) + e' 9 Cyt f(Fe2+) 0.385 NADP+ + 2e‘ + 2H+ 9 NADPH + 3* —0.320 1. (30 points) (a) (3 points) Name this figure. What types 0 organisms perform these reactions? Z SOMN PUMWS c1\C 0‘62. 01am; hXCK’e/ucxgég +2. ?v\ofu S 10mm“ cw?) am WM) (b) (3 points) What exactly is accomplished by cyclic electron flow?,P{cCMAL¢9 CA llJ-q'aivfl“_ Uszd 'er club/<2 ATP <24 WWwL/a ” (c) (10 points) Draw the structure of plastoquinone (PQ) and show its two—step reduction to plastohydroquinone (PQHZ). Indicate movement of electrons with appropriate arrows; you may draw just the parts of the PQ molecule undergoing change. "\Y‘- ‘ 1 q“\, “\'~ I ‘ C. V v T 7 I If azch wafflsz CH1) \ 2% t . ‘ \\\V d~ Z {4 I: EZ—N Na; K I 7' \a r K G /’_~k‘_v Cja I 0 \JA"// W“ A u __ f’ N \ (Xv « cw 5 6/) LIA/\ng _, / (d) (14 points) Calculate the AG°' for the reduction of PQ by water. What wavelength of light would provide enough energy to drive this reduction? How many quanta would be required for this reductio ? CI 7 a“ 4 I A \ «u‘ Z {UL ‘r’eciyé'hC-Yl Cbe’ 4 26 e Z“ ) 5; 052+ ‘(J‘DDL'I a, _ , »_ - _‘“"m“ 2 \ ~- +0 Ha" A5- +o.oza-~ 08‘s ,«032, M26 63 he?" :w—L-m kjm.1<”‘.\/“) <*O.’+3?>V) +(,.C282_\/ J, .5 l : '+ HUB K3 «MA L l *1 V” A guy: CEL‘LAA (4 .4 ‘3 EVE? bk 9‘ mduhm' -- /h\ ‘ ; :_ ' mix .l. “bx \‘ " ‘ \y L ‘5 k 3 f KICK e If“) ’ j y K ’ ‘ a a.“ -‘-—-—-—~.V , w . kw»; ,: 2.35 z< no 2 kg ; hf I is ‘23 q 1 N v ‘ )\ - 6 v ‘\ _ I : gm Harv-‘6) ( 3 mo m. 5 1 W5 "LQW ; MS m» m CY m5 and J ' — 238' x to” Name______l__Afl§Q£C_K—Q_‘a.l7___ 2 2. (28 points) (a) (2 points) The sea slug Elysia chlorotica steals chloroplasts from its algal food source, vaucheria litorea. The captured chloroplasts (kleptoplasts) remain functional in cells lining the sea slug's expansive digestive tract. The slugs develop a dark green color, subsist photoautotrophically and reproduce in culture, apart from any additional algal food source, for the duration of their normal 9 to 10 month life span. These "solar powered sea slugs” are able to fix “C—coz. What chloroplast enzyme would allow the slug to fix CO2 ? (,5 - RlbUlO$Q bxsphosphmi Carbox 5,0051; 028%QMQQ 0Q KuthCo (b) (8 points) Show how the 14C—product generated by the enzyme in part (a), would be used to synthesize glucose. What carbons of glucose would be labeled with 14C? (No electron pushing or mechanisms are required here!) show how we :5 [oWer-EJ +0 Hume @ fwm/ meg/H QI’IUM/ Carlee-Hg C3 My? C4 0*? /0{L€/F¢/ 6? gm?” \- +th5 1‘) o “ , _ (“420905 cHt- W3 P‘ CHLOWS— ch-{zop/J‘ S/IWIV) 1+ ' , ,_ C 0H 7 __L) y] c)h¢”‘z"w ’coo /&‘”P"3= Ab Ké~ (c;V(10 points) Describe (y uf’filay use egheehrm 6/1? truc ures) boa/the process of gluconeogenesis would be different in the sea slug before kleptoplasty (that is, before it captured the chloroplasts). How would it be similar? oqeeemwe some G9 “5‘3 W“ 0" [WWW @ms 74% 3106- flj/ufoJe @D Pym/We 90M -—7 pay a 2)? 6 -—) spa 6:; MM #4 0,6,1? «(e/rage Immi¥ Mom (d) (8 points) What pathway is required for gluconeogenesis in a germinating plant? Do you think the "solar—powered" sea slug would also have this pathway? Why or why not? GlyWyche rofihn/aj /C/c(€ 6,9 glue Tie Wagith lack 'H’Ieje enzym: Lemme) +he/ m: 710-4 [Mfr/If M CMOWF‘uS-flg (1+ 6m) 5411b Ch/lnf/ajy or, 5/95 flapabg ago hut WU 0Y1 (Ia/firm wsw’ (LS 5191mm g em?ij 7L9] Name__.;g\iq2%__EAv-I___.___ 3 4. (32 points total) For each of the following molecules that we have seen in class so far, write out the portion of the structure that is relevant to its biochemical role. The give an example of a reaction where we have seen it participate. You may either name the enzyme, or write out the substrates and products. If no enzyme is involved, then indicate the reaction with either names or structures of substrates and products. Molecule Relevant structure for function Example of reaction H /”‘ A K‘ /_ik V . ‘V ‘ r I} NAD+ /k\_ <;:§fj mflch LC%(QLKK (;:E?;>‘acfake (&fl\fidfiCffigrqu€ \ j V/,,« "YLL, , . Jf r’{JL /‘ ,‘ , \6' "A med” aflZ-‘lw‘tfl (”‘ (ZN'h 0’ ~“ fl ~ on ~ ', n Biotin )\ ‘ (WC-{W ‘ (“wig @(Locxleuag H W Nu F l x \ 43g:,’3 4%] I‘flfiflm‘ chm (up 5L1 ihfl \“flk’afl NS—methyl—THF QF<\VU/,.R\\ i l 2) N; "“‘ 1:1 \ ¥\ ‘ .l 9 1 .-(v, Ascorbic acid C KLQMXMV \ (3% OK ,--/ )(T’LH (Vitamin C) /7 x ' 4/ x_ u 5V ' \ \é (mam/At A ‘ L3: O§CCCVX K [’3 C "" ‘( aw (ilk M. Alpha-tocopherol (3V\ C u,\ h I‘ f} [~11:7“pe—~1 lemii (Vitamin E) j\\ &3L[C ‘f:) 5? a, f «A ' ’ .:><"10 H x To“a AK/L'R ( \I < (v Lia FAD ' Sum \m‘é (“WV/w 4" \‘ I n,” acd (HQ Alhak \ l ’7 0Q / H «a Pyridoxal g“ (UTHVKK32LHVQ’f; /2( phosphate f/j\\ / bj \~:\ Thiamin f” pj/ . : ‘ pyrophosphate (fink ,hdna)¥fl \ (3H ...
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