153CProblemSetVI - Study Questions and Problems VI...

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Revised 1/5/09 - 1 - Study Questions and Problems VI Chemistry 153C Winter 2009 1. (a) How might you expect the glutamine synthetase activity to change in an E. coli cell during a period of rapid growth when there is a tendency toward rapid utilization of glutamine for biosyntheses and a decrease in its steady-state concentration in the cell? Indicate the molecular basis for the changes which you think would occur in the cell to influence the activity of glutamine synthetase under these circumstances. (b) Indicate the ways in which L-glutamine is used as a reactant in biosynthetic sequences in an E. coli cell. 2. Glutamate synthetase, which has been shown to occur in microbial species and higher plants, catalyzes the following reaction: NADPH + H + + L-glutamine + α -ketoglutarate 2 L-glutamate + NADP + As the name implies, this enzyme is thought to be responsible for the biosynthesis of glutamate in these organisms. (a) Give an indication of the manner in which the three substrates must be participating in the overall transformation catalyzed by glutamate synthetase. (b) Why might this reaction be considered a part of an energetically more favorable sequence for the synthesis of L-glutamate than the L-glutamate dehydrogenase reaction?
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153CProblemSetVI - Study Questions and Problems VI...

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