23-Neuron-glia_interaction_II_current_re

23-Neuron-glia_interaction_II_current_re - Neuron-Glia...

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Neuron-Glia Interaction Part II: Glia Promote Neuronal Synapse Formation
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Three Serial Papers From Ben Barres’ Lab: Pfrieger et al., Science, 1997 Ullian et al., Science, 2001 Christopherson et al., Cell, 2005
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Methods: Immunopanning to purify r etinal g anglion c ells. Culture purified postnatal (P8) RGCs in serum-free medium, plus BDNF, CNTF, and B-27 (mixture of neurotrophic factors). Low density (50-100 cells/mm 2 ). Culture purified RGCs on glial cells isolated from their target area superior colliculus.
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Synaptic Efficacy Enhanced by Glial Cells in Vitro Frank W. Pfrieger, * Barbara A. Barres Science. 1997 Sep 12;277(5332):1684-7. This work focuses on synaptic efficacy using electrophysiology recording.
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Fig. 1 Spontaneous synaptic activity, 5 DIV Density higher? 50% show synaptic activity 90% show synaptic activity Both frequency and amplitude of synaptic events recorded from RGCs increased in coculture with glia Glia Increase Spontaneous Synaptic Activity
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Fig. 2 No glia With glia Each square is for the frequency and average amplitude in one neuron Both oligodendrocyte and astrocyte are effective Both Frequency and Amplitude are Enhanced
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Burst activity Miniature EPSCs in TTX Synapse morphology is normal +Glia Miniature EPSCs Increased Silent synapse! Morphological
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23-Neuron-glia_interaction_II_current_re - Neuron-Glia...

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