Lecture 2 - Lecture 2 Promoter-RNA polymerase binds here...

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Lecture 2 Promoter-RNA polymerase binds here Inducer-lactose-IPTG lacI-repressor which binds operator-allosteric inhibition (trans) lacZ encodes β-galactosidase (LacZ), an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose . (Explain mutant) lacY encodes β-galactoside permease (LacY), a membrane -bound transport protein that pumps lactose into the cell. (Explain mutant) I-constitutive-changes binding site for DNA-negative control can’t bind Prokaryotic Regulation II Lac operon usually only expressed in the presence of lactose Non-induced-grown in glycerol: medium without lactos analog (IPTG) Induced-grown in glycerol medium with a lactose analog (IPTG) Partial Diploids Demonstrate cis and trans regulation Inducible, constitutive, dominant Trans-free-floating protein Cis-represent sequences to which proteins bind Super repressor-cannot bind inducer (binding site disrupted)—changes binding site for RNA polymerase? Is mutants encode repressors that cannot bind lactose-continuously bind the lac operator site so that it
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This note was uploaded on 08/26/2011 for the course ANTHRO 200 taught by Professor Gouzoulesandkonner during the Spring '11 term at Emory.

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Lecture 2 - Lecture 2 Promoter-RNA polymerase binds here...

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