Chromatography Gel filtration

Chromatography Gel filtration - (mol wt) Sephadex G-25 5000...

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GEL-PERMEATION CHROMATOGRAPHY Gel-Permeation Chromatography is a mechanical sorting of molecules based on the size of the molecules in solution. Small molecules are able to permeate more pores and are, therefore, retained longer than large molecules.
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Gel filtration Distribution of analyte between: Inner mobile phase(Bed Vol) Outer mobile phase(void vol) is a function of molecular size.
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Exclusion limit Useful range (excluded in void vol)
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Unformatted text preview: (mol wt) Sephadex G-25 5000 1000-5000 G-50 30 000 1500-30000 G-75 70 000 3000-70000 G-100 150 000 4000-150000 G-150 400 000 5000-400000 G-200 800 000 5000-800000 Gel filtration Void volume:- add blue dye, allow to run, till dye appears = void volume Gel filtration Gel filtration Gel filtration Applications: 1.Purification 2.Molecular mass determination 3.Concentrating solution 4.Desalting...
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Chromatography Gel filtration - (mol wt) Sephadex G-25 5000...

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