Chromatography intro

Chromatography intro - Chromatography is a technique for...

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Unformatted text preview: Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or components ( Russian botanist Mikhail Semyonovich Tsvet 1906) . In 1952 Archer John Porter Martin and Richard Laurence Millington Synge were awarded the Chemistry Nobel Prize for their invention of partition chromatography Separate Analyze Identify Purify Quantify Components Mixture Uses for Chromatography Chromatography is used by scientists to: Analyze examine a mixture, its components, and their relations to one another Identify determine the identity of a mixture or components based on known components Purify separate components in order to isolate one of interest for further study Quantify determine the amount of the a mixture and/or the components present in the sample Uses for Chromatography Real-life examples of uses for chromatography: Pharmaceutical Company determine amount of each chemical found in new product Hospital detect blood or alcohol levels in a patients blood stream Law Enforcement to compare a sample found at a crime scene to samples from suspects Environmental Agency determine the level of pollutants in the water supply Manufacturing Plant to purify a chemical needed to make a product Simplified Definition : Chromatography separates the components of a mixture by their distinctive attraction to the mobile phase and the stationary phase. Explanation : Compound is placed on stationary phase Mobile phase passes through the stationary phase Mobile phase solubilizes the components Mobile phase carries the individual components a certain distance through the stationary phase, depending on their attraction to both of the phases Definition of Chromatography Compound is placed on stationary phase Mobile phase passes through the stationary phase Mobile phase solubilizes the components Mobile phase carries the individual components a certain distance through the stationary phase, depending on their attraction to both of the phase Definition of Chromatography Definition of Chromatography Detailed Definition: C hro m a to g ra phy is a la b o ra to ry te c hniq ue tha t s e pa ra te s c o m po ne nts within a m ixture b y us ing the diffe re ntia l a ffinitie s o f the c o m po ne nts fo r a m o b ile m e dium a nd fo r a s ta tio na ry a ds o rb ing m e dium thro ug h whic h the y pa s s ....
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Chromatography intro - Chromatography is a technique for...

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