Chromatography Ion exch

Chromatography Ion exch - (especially polyvalent) in eluant...

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Gradient elution useful (also for ion-exchange and partition chromatography)
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Ion-Exchange Chromatography Retention by attraction between groups on stationary phase with opposite charge to sample molecules. Stationary phase = insoluble, but solvent permeable polymer matrix ( eg cellulose) chemically modified to introduce ionizable groups ( eg -COOH). Elute by: Change of pH to neutralise charged group on either solute or stationary phase. Increase [salts]
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Unformatted text preview: (especially polyvalent) in eluant buffer --> Displace by competing ions. pH or salt gradient to enhance separation. Ion-Exchange Chromatography Ion-Exchange Chromatography Steps: 1.Equilibrium: ion exchanger & stationary phase in equilibrium 2.Sample application & wash: sample in same conc. /pH 3.Elution by changing ionic strength/pH 4.Regeneration to remove all molec. Ion exchange chromatography Ion exchange chromatography...
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This document was uploaded on 08/27/2011.

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Chromatography Ion exch - (especially polyvalent) in eluant...

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