Chromatography LC

Chromatography LC - LIQUID COLUMN CHROMATOGRAPHY LIQUID...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: LIQUID COLUMN CHROMATOGRAPHY LIQUID COLUMN CHROMATOGRAPHY A sample mixture is passed through a column packed with solid particles which may or may not be coated with another liquid. Glass column fused with porous plate/stopper/capillary tubing leading to detector and/fraction collector. LIQUID COLUMN CHROMATOGRAPHY Commonly used matrix are: 1. Agarose : polysaccharide made up of D-galactose & 3,6- anhydrogalactose units (gel permeation) 2. Cellulose : polysaccharide of β-1-4 D-glucose units (ion- exchange) 3. Dextran : polysaccharide of α-1-6 D-glucose units (gel permeation) 1.Polyacrylamide : acrylamide polymer (gel permeation) 2.Polystyrene : styrene polymer (ion-exchange) 3.Silica : polymer of orthosilicilic acid (gel permeation) LIQUID COLUMN CHROMATOGRAPHY Imp features of matrices are: • High mechanical stability • High chemical stability • Availability (range) ** • High capacity (functional groups) • Inert surface (avoid non- selective adsorption) ** 1. SEPHADEX G-10 2. SEPHADEX G-100 3. SEPHADEX G-100 SUPERFINE 4. SEPHADEX G-200 5. SEPHADEX G-25 COARSE 6. SEPHADEX G-25 MEDIUM 7. SEPHADEX G-25, FOR GEL FILTRATION 8. SEPHADEX G-50 9. SEPHADEX G-75 10. SEPHADEX LH-20 11. SEPHADEX-DEAE A-25, ION- EXCHANGE RESIN, 40-120 MICRON 12. SEPHADEX-DEAE A-50, ION EXCHANGE RESIN, 40-120 MICRON 13. SEPHADEX-SP, C-25, ION- EXCHANGE RESIN, 40- 120MICRON 14. SEPHADEX-SP, C-50 LIQUID COLUMN CHROMATOGRAPHY • Stationary phases: depending upon requirement • Particle size: larger size: faster flow rate, smaller particle: larger surface area, greater resolution • Column packing: slurry filled in, no air bubble, flow mobile phase, put nylon gauze on top avoid surface disturbance, always wet....
View Full Document

This document was uploaded on 08/27/2011.

Page1 / 59

Chromatography LC - LIQUID COLUMN CHROMATOGRAPHY LIQUID...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online