Chromatography Partition Adsorption

Chromatography Partition Adsorption - Definition: The...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
Definition: The analytical separation of a chemical mixture (gas or liquid) by passing it over an adsorbent bed that adsorbs different compounds at different rates. Involve weak non-ionic attractions like: van der vaal, H-bonding.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Adsorption chromatography Adsorbands : Silica (Si-OH) acidic good for basic materials Alumina basic, good for acidic materials Eluents (Polar comparable to analytes) Alcohols if analytes have OH groups Acetone/esters if analytes have –COOH gp Hydrocarbons (hexane/toluene) for non-polar Suited for non-ionic, water insoluble compounds like triglycerides, vitamins, drugs etc
Background image of page 2
Adsorption chromatography Hydroxylapatite chromatography (Ca 10 (PO 4 ) 6 (OH) 2 increase conc of buffer– ss desorbes further increase ds desorb Hydrophobic interaction chrom. (HIC) to purify proteins Hydrophobic groups masked by water layers Protein bind with matrix coated with alkyl/phenyl gps decreasing salt conc elute proteins
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 4
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Principles of Paper Chromatography Capillary Action
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This document was uploaded on 08/27/2011.

Page1 / 23

Chromatography Partition Adsorption - Definition: The...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online