Chromatography TLC affinity

Chromatography TLC affinity - soluble in the liquid move...

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Affinity chromatography
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Affinity chromatography Depends upon reversible adsorption of biomolecules on to ligand attached to matrix Ligands Affinity 5’ AMP NAD dependent dehydrogenases & some kinases Calmodulin Calmodulin binding proteins Lysine rRNA Fatty acids Fatty acid binding proteins
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Affinity chromatography Steps: 1. Equilibrium 2. Sample application & wash 3. Elution
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Affinity chromatography
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T hin L ayer C hromatography Here the mobile phase is a liquid Flowing past a thin layer of powder on a solid support. Substances that are less attracted to the solid or are more
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Unformatted text preview: soluble in the liquid move faster. And so move further up the plate by the time that the process has been stopped by taking the plate out of the liquid. - larger Rf Establishing the Pre-Equilibrium of a TLC Plate R f = distance moved by substance distance moved by solvent front For substances that are very soluble in the liquid R f will be close to .... For substances that are rather insoluble in the liquid R f will be close to .... 1...
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This document was uploaded on 08/27/2011.

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Chromatography TLC affinity - soluble in the liquid move...

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