Thin Layer Chromatography

Thin Layer Chromatography - Andre Hinojosa 12601675...

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Unformatted text preview: Andre Hinojosa 12601675 Partner: Chelsea Azevedo 7/7/10 Thin Layer Chromatography The purpose of this experiment was to use thin layer chromatography techniques to determine what compounds are in a mixture as well as identify unknowns. Although we compared calculate Rf values to find out the identity of an unknown analgesic, we also used these techniques to determine the purity of our caffeine samples from our last lab. The TLC plates were difficult to work with because the top was layered with a thin, delicate, white powdery solid that crumbled easily when too much pressure was applied. This made marking solvent fronts, base lines, and circling spots difficult. We noticed that if the baseline crumbled, it had a big impact as the solvent rose through the plate, leaving a dry section where the plate broke. In our first plate with the 10:4:1 mixture, the spots were very difficult to see. After the solvent evaporated from the plate we left it in an iodine chamber with powdered iodine for about...
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Thin Layer Chromatography - Andre Hinojosa 12601675...

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