forensictoxmod3 - Module 3 Page 1 of 8 Printable View of:...

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Print Save to File Printable View of: Module 3 File: Module Overview Module Overview Module 3: Immunoassays Introduction Hello and welcome to Module 3 - Immunoassays. In this module we will introduce you to the principles, advantages, and disadvantages of several different immunoassay techniques used for the identification and/ or quantification of drugs of abuse in body fluids such as blood, plasma, and urine. Objectives At the end of this module students should: 1. Understand the chemistry and conceptual principles of immunoassay techniques as they pertain to forensic drug analysis and forensic toxicology. 2. Understand the applications, advantages and disadvantages of the different immunoassay techniques utilized for the analysis of drugs and their metabolites in body fluid specimens. 3. Understand the processes involved in Radioimmunoassay (RIA), Enzyme Multiplied Immunoassay Technique (EMIT), Enzyme Linked Immunoabsorbent Assay - (ELISA), Fluorescence Polarization Immunoassay - FPIA (ADx) and Cloned Enyzme-Donor Immunoassay (CEDIA). 4. Understand the sample preparation required for testing samples using immunoassay techniques. 5. Understand the need for controls, and calibrators in the assay technique. 6. Understand the terminology associated with immunoassay techniques. Assignment Please note: The assignment is listed below and again on the final page of this module. You should consider the assignment questions as you read through the module content, then submit your assignment using the assignment submission form on the final page of this module. 1. Develop a summary (about 500 words) of pharmacological effects and blood or plasma drug levels associated with recreational use, and overdose, for one commonly abused drug of your choice. 2. How do you think immunoassay cross-reactivity could be used to our advantage (if it can)? Post your responses on the Bulletin board for Module 3. Page 1 of 8 Module 3 8/2/2009 ..
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File: Principles Principles Module 3: Immunoassays Principles Immunoassays are techniques used to measure the presence and amounts of a chemical substance in body fluids such as blood, plasma, serum, vitreous humor and more commonly, urine. These assays work on the principle of competition between a drug (analyte) present in the sample with a labeled drug or an antibody specific for the drug of interest. Assays need to exhibit specificity (the degree to which an assay correctly identifies a compound of interest.) and sensitivity (the assays ability to detect an analyte in a sample). Nearly all immunoassays are comprised of a few common elements that are briefly described below: 1. Antibodies - protein molecules of the immune response developed in animals for detection of a specific antigen 2. Antigens - molecules that elicit an immune response and bind with antibodies 3. A molecular label - may be a totally separate molecule from the antigen, or a modification of it
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forensictoxmod3 - Module 3 Page 1 of 8 Printable View of:...

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