ChromEphorW11 - Chromatography & Electrophoresis I....

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Bruce Heyer 1 Separation Techniques I. Chromatography II. Electrophoresis I. Chromatography Chroma : “color” + graph : “measure” –Developed in 19th century to separate pigments from plants and dyes –Refined technique now used to separate, • Still called “chromatography” even though no longer limited to separating colored molecules Chromatography Mobile phase : fluid solvent that flows over/through the stationary phase Stationary phase : gel, beads, or other solid medium with specific absorptive characteristics Mixture of mac rom ole cul e s olu tes from sample dissolved in mobile phase Separation of mac rom ole cul e s olu tes from mixture in original sample — based upon relative rates of migration through the stationary phase •Solutes that interact strongly with the stationary phase do not migrate very far •Solutes that interact with the stationary phase migrate slower than the mobile phase •Solutes that do not interact with the stationary phase migrate at the same rate as the mobile phase Solutes interact with stationary phase
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This note was uploaded on 09/02/2011 for the course BIOL 6B taught by Professor Heyer during the Spring '10 term at DeAnza College.

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ChromEphorW11 - Chromatography & Electrophoresis I....

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