LabTransform - Recombination may occur between donor...

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pGLO Transformation 1 Transformation with pGLO Plasmid HOW BACTERIA GAIN NEW GENETIC INFORMATION • MUTATIONS • RECOMBINATION –TRANSDUCTION –TRANSFORMATION –CONJUGATION • TRANSPOSITION TRANSFORMATION Cells after death, release DNA Naked DNA in solution Cells may take up DNA Only in certain stage of cell cycle Near completion of cell wall synthesis Competent cells-receptor sites on cell wall and plasma membrane Release competence factor that helps in uptake Alters cell wall Makes it more permeable to DNA Not E. coli — needs to be treated to be competent Endonucleases cut linear DNA into small pieces
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Unformatted text preview: Recombination may occur between donor & recipient-complementary base pairing Non-rcombined linear fragments are degraded Circular plasmid DNA protected from degradation TRANSFORMATION pGLO Transformation of E. coli Gene Expression Transcription & Translation pGLO Transformation McCauley & Heyer 2 Transcription starts at a promoter Regulation of an Operon The pGLO plasmid The pGLO plasmid The Arabinose Operon Negative or Positive gene regulation? Repressible or Inducible? Expression of GFP Without arabinose in media With arabinose in media...
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LabTransform - Recombination may occur between donor...

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