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Unformatted text preview: Recombination may occur between donor & recipient-complementary base pairing Non-rcombined linear fragments are degraded Circular plasmid DNA protected from degradation TRANSFORMATION pGLO Transformation of E. coli Gene Expression Transcription & Translation pGLO Transformation McCauley & Heyer 2 Transcription starts at a promoter Regulation of an Operon The pGLO plasmid The pGLO plasmid The Arabinose Operon Negative or Positive gene regulation? Repressible or Inducible? Expression of GFP Without arabinose in media With arabinose in media...
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