RestrictionDigest - DNA Digestion & Ligation Lab...

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1 RESTRICTION ENZYMES • Bacteria produce special enzymes to chop up viral DNA. • Biotechnologist use these “restriction enzymes” to cut DNA in specific places (restriction sites). • Many restriction enzymes cut the DNA polymer in a staggered pattern that produce “sticky” single-stranded ends to the DNA fragments. RESTRICTION ENZYMES • Since a particular restriction enzyme only cuts DNA at a specific DNA sequence (restriction site): • Any DNA cut by that enzyme will have the same “sticky
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