Bio47Lab02-1 - 2 Name Microscope Cytology Objectives 1...

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Lab 2 – Microscopy Rev. 4 Bio47 - Human Anatomy 1 2 Name : ____________________________________________ Microscope / Cytology Objectives : 1. Understand the difference between Magnification and Resolution 2. Identify the major microscopic components & demonstrate proper microscope use 3. Define and describe cellular structure 4. Describe structure and function of cellular organelles. 1A. Microscopy : " The evolution of a science often parallels the invention of instruments that extend human senses to new limits ." (Campbell 2002) The microscopic study of anatomy is an excellent example supporting this statement. Without the aide of microscopes, our our study and acquisition of knowledge about organisms and cells cells smaller than 0.1 mm (limit of the human eye) would never have never have been possible. The first microscope constructed and used and used for the examination of cells was the light microscope in in 1665 by Sir Robert Hooke. He utilized the magnifying power of power of the simple yet powerful light microscope to describe for the for the first time the general structure of cells, symmetrical repeating repeating patterns of cubes which reminded him of small monastery monastery rooms called cells, hence the name. This began the wondrous journey into the unknown world of cells, launching the the field of study known as Cytology . Light microscopes function by function by passing visible light through a specimen & series glass glass lenses. The lenses function to bend & change the course of the of the light as it passes toward the eye. The result is the production production of a magnified specimen image. Light microscopes are are effective in magnifying images up to 1000x. Even today, the the light microscope is the most commonly used scientific instrument. It is for this reason that anyone entering into scientific scientific study should become familiar with the use and care of of microscopes. Remember, just as the invention of instruments can instruments can extend our human senses, the ignorance of their their proper use can hinder scientific discovery. 1B. LIGHT MICROSCOPY : Two important parameters of microscopy are : 1. Magnification : The enlargement of a specimen’s image size beyond actual size or the ration of an object’s image to it’s actual size 2. Resolving Power (Resolution) : A measure of the images clarity; it is the minimum distance that two points can be clearly seen as distinct and separate points. The limiting factor of most microscopes is the resolving power. For example, light microscopes use light to illuminate images. The smallest wavelengths of visible light are too large to resolve images smaller than 0.25 micrometers (um). This means that when images are enlarged greater than 1,000x,
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Lab 2 – Microscopy Rev. 4 Bio47 - Human Anatomy 2 they will start to appear blurry. Therefore, the greater the magnification, the lower the resolving power. In order to clearly see objects smaller than 0.25um, a media with a much smaller wavelength must be
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Bio47Lab02-1 - 2 Name Microscope Cytology Objectives 1...

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