CBP 1 Cell organelles SOUL

CBP 1 Cell organelles SOUL - CBP 1 Cell organelles SOUL

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CC/LR/CBP There are two sorts of cells, In this course we concentrate on eukaryotes C We will start by defining The Cell C and then proceed to examine cell organelles and their various functions.
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CC/LR/CBP 1: The Entropy Problem Entropy can be considered as disorder in a system Disorder occurs spontaneously Order requires energy In our universe for any spontaneous process the entropy must increase. (2 nd Law of Thermodynamics) Increase in disorder causes an increase in entropy Life is highly organised Requires a lot of energy Metabolism must be fast
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CC/LR/CBP 2: Mass Transport The movement of substances DIFFUSION C only solute shows net movement C no net movement of bulk phase (solvent) C movement random (down concentration gradient) CONVECTION C C vectorial C rate depends upon input of energy
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CC/LR/CBP But surface to volume ratio is not the only problem; Think nerve cells?
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CC/LR/CBP 3: Eukaryote Cell Cytoskeleton Three Structural Elements, C All protein fibres made up of monomer sub-units Microfilaments, C monomer a globular protein~Actin Microtubules, C monomer a globular protein ~Tubulin Intermediate filaments, C monomer a long chain protein ~IFP
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CC/LR/CBP
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CC/LR/CBP 4: Cytoskeleton Functions Some functions could be inferred from the structure and distribution of cytoskeleton. C Provides a scaffold or framework C Maintain arrangement of internal organelles C Control external shape of cell Better understanding of the dynamic nature of some cytoskeleton components required the development of new microscopic techniques. Particularly using fluorescence
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CC/LR/CBP ACTIN FILAMENTS INTER MEDIATE FILAMENTS MICROTUBULES Visualised using anti cytoskeleton fluorescent labelled antibodies
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CC/LR/CBP 5: Fluorescence Microscopy Specimen irradiated by intense light Light absorbed by the chromophore is re- emitted as light of a longer wavelength C Fluorescence is very weak C Irradiating wavelengths are excluded by filters C So that only re-emitted light is viewed Zero background = high contrast
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This note was uploaded on 07/04/2011 for the course BIO microbiolo taught by Professor Point during the Spring '09 term at HKU.

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CBP 1 Cell organelles SOUL - CBP 1 Cell organelles SOUL

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