CBP 2 Membranes SOUL

CBP 2 Membranes SOUL - CBP 2 Membranes SOUL

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CC/LR/CBP 1: What makes a membrane? Overton, C Permeability of membranes C Examined penetration of dyes for stains into intact cells C Easily penetrated by lipophilic substances C Impermeable to hydrophilic substances C Conclusion, membranes have a lipid character C Thermodynamic problem? Lipid and water don’t mix.
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CC/LR/CBP 2: Langmuir’s Trough Investigation of intermolecular forces C Langmuir trough, water tank to provide flat surface C Use a drop of lipid to spread a monomolecular film, C Langmuir found the best films were formed with phospholipids from nervous tissue C Movable barriers on the water surface compress the film and the force required for reduction in area can be measured.
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CC/LR/CBP Langmuir Trough Torsion gauge to measure force on boom
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CC/LR/CBP Langmuir’s Results
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CC/LR/CBP Phospholipid bilayer
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CC/LR/CBP 3: Do cells have enough phospholipids? C Acetone extraction of erythrocyte lipids. C Measured the area of a monomolecular film produced using the extracted lipids on a Langmuir trough. C Calculated the area of monolayer produced by quantity of lipid corresponding to a single cell. C Why can this experiment only work with erythrocytes?
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CC/LR/CBP 4: Is the bilayer big enough? Area of erythrocyte cell surface 100µm 2 Area of the monolayer film was 200µm 2 Yes, it is consistent with phospholipid bilayer covering the cell surface. Thickness calculated to be around 5 nm
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CC/LR/CBP 5: What else is there in a membrane? Do you believe in ghosts? How to make a ghost…… Of a rbc! Repeated washing in water until no colour in supernatant after centrifugation Extract pellet and analyse composition
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CC/LR/CBP 6: Where’s the protein? Composition of erythrocyte membrane from
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This note was uploaded on 07/04/2011 for the course BIO microbiolo taught by Professor Point during the Spring '09 term at HKU.

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CBP 2 Membranes SOUL - CBP 2 Membranes SOUL

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