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Updated 4/9/11 1 THE MICROBIOLOGY LAB PRACTICAL STUDY GUIDE The practical will be given on April 18/19 th , during your lab session. The lab practical will consist of about 25 stations, one station set up at each seat in the lab room. Each station will have something for you to look at, interpret or identify, or a short problem or procedure for you to do. The practical is limited to material covered in lab or in your lab manual. You will spend two minutes at each station and then move on to the next one. You will have a few minutes at the end of the practical to check over your answers, but you will not be allowed to return to any of the stations. Therefore, we encourage you to take notes while at each station. IN GENERAL: Safety procedures (when and why to wear lab coats, gloves, safety glasses, etc.) Proper disposal of materials (petri plates, test tubes containing media, slides, etc.) Labeling procedures - what information do you need to put on a petri plate or test tube? Be able to identify a pure vs. mixed culture of bacteria. Be able to suggest the starting steps to identifying an unknown organism growing on a petri plate. Be able to do problems involving dilutions ( e.g., if you have a 10 -3 dilution with 10 cells/mL, what was the concentration of the stock solution and vice versa). Know which organisms used in lab are prokaryotes and which are eukaryotes. UNDERSTAND CONTROLS: What are controls? How would you set up a control for an experiment? What are positive and negative controls? Why do we use controls? (Also see Appendix II in your lab manual) A control is an integral part of any well-designed experiment. Control elements are set up exactly like experimental elements, except you know the outcome of the control variable you are testing. A positive control is something you know will react positively, so you can compare it to your experimental result. For example, a motile bacterium would make a good positive control for a motility test of an unknown strain. Negative controls let you know whether or not you can trust your experimental results and what aspects in the experiment may be affecting them, such as contamination, problems with chemicals, etc. Another type of negative control is something you know exhibits a negative reaction, such as a non-motile bacterium. TOPICS COVERED: As a reminder, these are the labs and concepts we covered that are ‘fair game’ for the lab practical:
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Updated 4/9/11 2 LAB 1 – Media preparation & sterilization: Recognize the media types (broth, agar deeps, agar slants). The autoclave: Why do we autoclave media? Equipment? Biohazardous waste? What are the different types of media used for? What is defined media vs. undefined media? What is the function of agar? Why do we add the other ingredients? LAB 2
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This note was uploaded on 09/03/2011 for the course BME 233 taught by Professor Izatt during the Spring '11 term at Duke.

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