QuestionSet1_early embryonic transcription

QuestionSet1_early embryonic transcription - BIO 295s Genes...

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Early transcription in Caenorhabditis elegans embryos Edgar et al, 1994 1) Intro - What developmental processes did genetic analysis of C. elegans suggest are under maternal vs. embryonic control? Early embryogenesis occurs when many unequal and asynchronous cleavages result in unique founder cells for primary somatic lineages. 2) Following labeling with radioactive UTP, why did they treat with DNase, proteinase and RNase? Four aliquots, one untreated and the others treated with either DNase, proteinase and RNase respectively allowed determination of the fraction of radioactivity attributable to RNA. Most of the incorporation of [ 32 P]UTP was in RNA. 3) Alpha-amanitin inhibits RNA Polymerase II. Why was it important for the authors to compare radioactive labeling in the presence and absence of alpha-amanitin? Alpha-amanitin has little to no effect on RNA Polymerase I and III. This allows us to determine relative contribution of RNA Polymerase II to transcription of mRNA. 4) Why were they counting silver grains?
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This note was uploaded on 09/03/2011 for the course BIOLOGY 295 taught by Professor Baugh during the Spring '11 term at Duke.

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QuestionSet1_early embryonic transcription - BIO 295s Genes...

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