Chem 152 abs spect lab report

Chem 152 abs spect lab report - Absorption Spectroscopy...

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Absorption Spectroscopy Author: Lab Partners: Instructor: Chem 152, Monday Lab Date Report Submitted: 08/29/10 Abstract: This experiment’s purpose is to use Beer’s Law (A = εbC) to determine epsilon (slope) of a stock dye called Red40 which has an initial molarity of 3.989*10^-3M. Epsilon was determined to be 4.57*10^4 L/mol*cm from our 4 four standards prepared from the Red40 dye using the parallel dilution technique. The absorption measurements (determined from absorption spectroscopy) were graphed versus their concentrations to determine the slope (ε).
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Introduction By using absorption spectroscopy, one will learn how to measure absorbance’s, perform a Beer’s Law analysis to determine a solutions concentration, and then the capability of finding a line of best fit from the plotted absorbance measurements and concentrations to get ε (the slope). Chemical reactions are governed by the number of molecules involved in a reaction, but atoms and molecules are so small they can’t be counted directly so a more complex technique must be used. Spectroscopy is a great technique to use because it provides a way to make direct
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Chem 152 abs spect lab report - Absorption Spectroscopy...

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