Review Sheet, Prelim 1 - BME 3020 Spring 2011 Prelim...

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Unformatted text preview: BME 3020 Spring 2011 Prelim #1 Review Sheet Lecture 2 – Fundamentals of Cell Biology • • • • • • What are characteristic features of cells in general? Know the approximate size of cells and their components. What is the difference between prokaryotes and eukaryotes? What are the 4 major structural features of eukaryotic cell? Know the structure and function of the cell membrane. How does fluorescent antibody staining work? What is the difference between primary and secondary antibodies? Lecture 3 – Cell Culture Basics • • • • • • What is tissue culture (different from organ and cell culture?)? Why do we do tissue culture? What can be studied with tissue culture? What are the advantages and disadvantages of tissue culture? Know about cell isolation – what is it? Why is it done? Know the different methods that can be used to do it. How are cells maintained in culture? o Absolute requirements o Requirements for general cell metabolism o Insoluble factors – adhesion matrix What is the difference between insoluble and soluble factors? Be able to give examples of both. What is cell growth? Lecture 4 – Cell Culture Techniques • • • • • • What is anchorage ­dependence? Be able to name the types of cells that are substrate ­independent, What are the advantages of 3D culture over monolayer culture? Be able to give some examples of 3D cultures What is a feeder layer? What does a typical growth curve look like? Lecture 5  Receptor Ligand Binding • • • • • • What is a ligand? What types of responses can we get with ligand binding? Can the same ligand bind multiple receptors? What is biding specificity? What is effector specificity? What are the 3 receptor classes? Know them well. What experimental techniques can be used to study binding? BME 3020 Spring 2011 Lecture 6 – Receptor Ligand Binding (mathematical models) • • • • • • What assumptions are made in the monovalent binding model? What is a Van’t Hoff Plot? What can you determine from a Van’t Hoff Plot? What is a Scatchard Plot? Understand the rate constants and the equilibrium constants. What is special about heat capacity of proteins? Why? Lecture 7 – Receptor Ligand Binding (medical perspective) • • • Understand how anthrax, cholera, etc. cause infections What are gram ­negative bacteria? What are the symptoms of cholera? Homework 1 • • • What are pluripotent vs multipotent stem cells? What are the advantages and disadvantages of iPS cells? What are feeder layers? Why is it used? Homework 2 • • • What’s the structure of integrins, and how does it contribute to specificity? What is bidirectional signal transduction? What is receptor crosstalk? What is the difference between full ­length fibronectin (FN) and FN fragments in terms of specificity? ...
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