MicrotechniqueFinal - Microtechnique Final Exam Chris...

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Microtechnique Final Exam Chris Phelps 11.24.06
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1.) The ASA or ISO number attributed to film describes the size of the emulsion grain on that film. The emulsion grain is the silver halide which oxidizes on the surface of the film in the presence of light. The larger the grain on the surface, the faster the film will be exposed. With smaller grain on the surface, you excellent picture quality, but it requires a long period of exposure. In photo microscopy, the light conditions are constant, so choosing a film based on changing light is unnecessary. When choosing a film for use in photo microscopy, you must compromise. For example, with a choice between three types of film, Pan-X (ISO 32), Plus-X (ISO 100), and Tri-X (ISO 400), each has its own benefits. The Pan-X will produce immensely detailed photos, but it requires quite a long time for exposure. The Tri-X film will expose very quickly, but since the emulsion grain is so large, the pictures will look grainy and lack detail. To capture the best pictures, the compromise is to take the medium Plus-X film. The emulsion grain is medium, so the pictures are relatively detailed while keeping the exposure time down. 2.) Picric Acid : explosive, irritant, toxic, flammable Sodium Hydroxide : caustic Hydrochloric Acid : corrosive Xylene : irritation of the skin, eyes, nose, and throat; difficulty in breathing; problems with the lungs; delayed reaction time; memory difficulties; stomach discomfort; and possibly changes in the liver and kidneys Methanol : flammable, toxic Ethanol : flammable
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3.) Lipid Stains: Nile Red, Nile Blue, Osium Tetroxide, Sudan Stains
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This note was uploaded on 04/05/2008 for the course BIOL 3550 taught by Professor Robertson during the Spring '08 term at East Tennessee State University.

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MicrotechniqueFinal - Microtechnique Final Exam Chris...

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